Green validated chromatographic methods for simultaneous determination of co-formulated oxytetracycline HCl and flunixin meglumine in the presence of their impurities in different matrices

Abstract Green analytical chemistry is an important area of chemical sciences. So, developing competent eco-friendly tools a big challenge. This study devotes two chromatographic techniques for the simultaneous determination binary formulated mixture oxytetracycline HCl (OXY) and flunixin meglumine (FLU), their impurities tetracycline (TRC) 2-chloronicotinic acid (CNA), respectively. Primarily, TLC densitometric method proposed validated using plates sprayed with 5% EDTA pH 9 as stationary phase (acetonitrile/ distilled water/ ethanol 7:2:1, by volume) system. Well-separated spots are detected at 267 nm where linear relations have been achieved 0.05–2 μg/band 0.01–2 OXY FLU, The second developed HPLC which fulfilled on Hypersil BDS column-C18. A mobile water 0.1%TFA/ acetonitrile in ratio 82: 18, v/v firstly then 70: 30, after min used sequential isocratic elution 210 FLU OXY, TRC, CNA. Sensitive wide linearity ranges 0.05–200 μg/mL both drugs. methods applied successfully cattle meat milk cited drugs determination. According to ICH guidelines, validation has accomplished methods. Statistical comparison carried out official reported Eventually, greenness procedures evaluated Eco-Scale gives results. Graphical abstract